Relative Evaluation of the Application of Polystyrene Microspheres and Polystyrene Carboxyl Microspheres in Biotechnology – Concentrating On Nucleic Acid Removal.
(LNJNbio Polystyrene Microspheres)
In the field of modern biotechnology, microsphere products are extensively used in the extraction and filtration of DNA and RNA because of their high certain area, good chemical security and functionalized surface area properties. Among them, polystyrene (PS) microspheres and their obtained polystyrene carboxyl (CPS) microspheres are just one of both most extensively studied and used materials. This short article is given with technical support and data analysis by Shanghai Lingjun Biotechnology Co., Ltd., aiming to methodically contrast the performance differences of these two types of products in the procedure of nucleic acid removal, covering essential indicators such as their physicochemical residential properties, surface area modification ability, binding efficiency and recovery rate, and highlight their suitable circumstances via speculative information.
Polystyrene microspheres are uniform polymer particles polymerized from styrene monomers with excellent thermal security and mechanical strength. Its surface is a non-polar structure and usually does not have energetic useful groups. As a result, when it is directly made use of for nucleic acid binding, it needs to count on electrostatic adsorption or hydrophobic action for molecular fixation. Polystyrene carboxyl microspheres present carboxyl practical teams (– COOH) on the basis of PS microspheres, making their surface area capable of more chemical coupling. These carboxyl groups can be covalently bound to nucleic acid probes, proteins or various other ligands with amino teams via activation systems such as EDC/NHS, thereby accomplishing much more secure molecular fixation. For that reason, from an architectural point of view, CPS microspheres have a lot more advantages in functionalization possibility.
Nucleic acid extraction typically includes actions such as cell lysis, nucleic acid launch, nucleic acid binding to strong stage carriers, cleaning to remove pollutants and eluting target nucleic acids. In this system, microspheres play a core duty as solid stage providers. PS microspheres generally depend on electrostatic adsorption and hydrogen bonding to bind nucleic acids, and their binding performance is about 60 ~ 70%, but the elution efficiency is reduced, just 40 ~ 50%. In contrast, CPS microspheres can not only utilize electrostatic effects however additionally accomplish even more solid fixation via covalent bonding, reducing the loss of nucleic acids during the cleaning process. Its binding performance can reach 85 ~ 95%, and the elution performance is also boosted to 70 ~ 80%. In addition, CPS microspheres are additionally substantially better than PS microspheres in terms of anti-interference ability and reusability.
In order to verify the performance differences between both microspheres in actual procedure, Shanghai Lingjun Biotechnology Co., Ltd. conducted RNA removal experiments. The speculative samples were originated from HEK293 cells. After pretreatment with common Tris-HCl buffer and proteinase K, 5 mg/mL PS and CPS microspheres were used for removal. The outcomes showed that the typical RNA return extracted by PS microspheres was 85 ng/ μL, the A260/A280 ratio was 1.82, and the RIN value was 7.2, while the RNA yield of CPS microspheres was enhanced to 132 ng/ μL, the A260/A280 ratio was close to the ideal worth of 1.91, and the RIN value got to 8.1. Although the operation time of CPS microspheres is a little longer (28 minutes vs. 25 minutes) and the expense is higher (28 yuan vs. 18 yuan/time), its extraction high quality is dramatically enhanced, and it is better for high-sensitivity discovery, such as qPCR and RNA-seq.
( SEM of LNJNbio Polystyrene Microspheres)
From the viewpoint of application circumstances, PS microspheres appropriate for large screening tasks and preliminary enrichment with reduced requirements for binding uniqueness as a result of their low cost and easy procedure. However, their nucleic acid binding ability is weak and conveniently impacted by salt ion concentration, making them improper for long-lasting storage space or repeated usage. In contrast, CPS microspheres are suitable for trace sample removal due to their rich surface area useful groups, which help with further functionalization and can be utilized to build magnetic bead detection kits and automated nucleic acid removal platforms. Although its prep work process is relatively complicated and the expense is reasonably high, it reveals stronger adaptability in scientific study and professional applications with rigorous requirements on nucleic acid removal efficiency and purity.
With the rapid development of molecular medical diagnosis, gene editing and enhancing, liquid biopsy and other areas, higher requirements are positioned on the efficiency, pureness and automation of nucleic acid extraction. Polystyrene carboxyl microspheres are progressively changing traditional PS microspheres due to their exceptional binding performance and functionalizable features, coming to be the core option of a new generation of nucleic acid extraction materials. Shanghai Lingjun Biotechnology Co., Ltd. is likewise constantly optimizing the bit dimension distribution, surface area thickness and functionalization efficiency of CPS microspheres and developing matching magnetic composite microsphere products to satisfy the demands of professional diagnosis, clinical study organizations and commercial customers for high-grade nucleic acid extraction remedies.
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Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding and more. We not only provide products but can also undertake OEM, ODM, and other needs. If you need dna extraction, please feel free to contact us at sales01@lingjunbio.com.
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